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A sensitive and selective immuno‐nanogold resonance‐scattering spectral method for the determination of trace penicillin G
Author(s) -
Jiang Zhiliang,
Li Yan,
Liang Aihui,
Qin Aimiao
Publication year - 2008
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1026
Subject(s) - penicillin , chemistry , detection limit , linear range , chromatography , resonance (particle physics) , hapten , citric acid , analytical chemistry (journal) , biochemistry , antibiotics , antigen , physics , biology , genetics , particle physics
A sensitive and selective immuno‐nanogold resonance scattering spectral assay was developed for the determination of trace hapten penicillin G, based on the resonance scattering (RS) effect of the nanogold at 560 nm, and the nanogold‐labelled immunoreaction took place in pH 5.4 phosphate citric acid buffer solutions and in the presence of polythylene glycol (PEG). The nanogold‐labelled immunocomplex formed more and more with addition of penicillin G. The enhanced RS intensity at 560 nm Δ I RS was linear to the penicillin G concentration in the range 7.5–1700 ng/mL, with a detection limit of 0.78 ng/mL. The results indicate that the immunonanogold‐labelled RS spectral assay has a high specificity and sensitivity for quantitative determination of penicillin G in raw milk samples. Copyright © 2008 John Wiley & Sons, Ltd.