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Protein synthesis in eukaryotic organisms: New insights into the function of translation initiation factor EIF‐3
Author(s) -
Hannig Ernest M.
Publication year - 1995
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950171103
Subject(s) - eukaryotic translation initiation factor 4 gamma , initiation factor , translation (biology) , eif4a1 , eukaryotic initiation factor , eukaryotic translation , protein biosynthesis , eif4ebp1 , function (biology) , biology , eif2 , computational biology , eif4e , microbiology and biotechnology , genetics , messenger rna , gene
The pathway for initiation of protein synthesis in eukaryotic cells has been defined and refined over the last 25 years using purified components and in vitro reconstituted systems. More recently, powerful genetic analysis in yeast has proved useful in unraveling aspects of translation inherently more difficult to address by strictly biochemical approaches. One area in particular is the functional analysis of multi‐subunit protein factors, termed eukaryotic initiation factors (eIFs), that play an essential role in translation initiation. eIF‐3, the most structurally complex of the eIFs, has until recently eluded this approach. The identification of the yeast GCD10 gene as the structural gene for the ζ subunit of yeast eIF‐3 (1) and the analysis of mutant phenotypes has opened the door to the genetic dissection of the eIF‐3 protein complex.