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The channels model of nuclear matrix structure
Author(s) -
Razin Sergey V.,
Gromova Irina I.
Publication year - 1995
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950170512
Subject(s) - nuclear matrix , dna , scaffold/matrix attachment region , computational biology , genome , biology , matrix (chemical analysis) , cleavage (geology) , genetics , nuclear dna , microbiology and biotechnology , biophysics , chemistry , gene , chromatin , paleontology , chromatography , fracture (geology) , chromatin remodeling , mitochondrial dna
The specificity of eukaryotic DNA organization into loops fixed to the nuclear matrix/chromosomal scaffold has been studied for more than fifteen years. The results and conclusions of different authors remain, however, controversial. Recently, we have elaborated a new approach to the study of chromosomal DNA loops. Instead of characterizing loop basements (nuclear matrix DNA), we have concentrated our efforts on the characterization of individual loops after their excision by DNA topoisomerase II‐mediated DNA cleavage at matrix attachment sites. In this review the results of applying this mapping approach are compared with the results and conclusions from studies of nuclear matrix DNA. An attempt is also made to reconsider all data about the specificity of DNA interactions with the nuclear matrix and to suggest a model of spatial organization of the eukaryotic genome which resolves apparent contradictions between these data.