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The formation and functioning of yeast mitotic spindles
Author(s) -
Masuda Hirohisa
Publication year - 1995
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950170110
Subject(s) - spindle pole body , spindle apparatus , multipolar spindles , spindle checkpoint , kinetochore , microbiology and biotechnology , chromosome segregation , biology , mitosis , microtubule nucleation , sister chromatids , microtubule , microtubule organizing center , genetics , chromosome , cell division , cell , centrosome , cell cycle , gene
The mitotic spindle contains the machinery responsible for sister chromatid segregation. It is composed of a complex and dynamic array of microtubules, which are nucleated from the spindle poles. Studies of yeast spindle functions by molecular genetic analysis and by in vitro functional analysis have identified proteins that are mitosis‐specific and present at very low concentrations in the cell, and have revealed the molecular bases of several processes required for the formation and functioning of the mitotic spindle. Here I review the current knowledge of the processes that are common to most eukaryotes: microtubule nucleation at the spindle poles, bipolar spindle assembly, maintenance of the spindle structure, chromosome attachment to the spindle and chromosome separation on the spindle.