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TKO'ed: Lox, stock and barrel
Author(s) -
Chambers Cynthia A.
Publication year - 1994
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950161202
Subject(s) - homologous recombination , mutant , genetics , biology , recombination , gene , in vivo , bacteriophage , homologous chromosome , gene targeting , mutation , computational biology , escherichia coli
The generation of panels of mutant mice by homologous recombination has greatly increased the ability to assess the function of particular gene products in vivo. The ability to control the developmental stage, the tissue and the nature of the mutation would be an important innovation. A recent report (1) demonstrates that the conservative site‐specific recombination of bacteriophage P1, namely Cre‐ lox , can be used successfuly in combination with homologous recombination to generate temporal‐and cell‐restricted mutations in vivo. The technical advance allows a greater flexibility in gene targeting and will have a significant impact on how complex gene functions are studied in vivo .