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Genes and genomes: Sequencing 5‐methylcytosine residues in genomic DNA
Author(s) -
Grigg Geoffrey,
Clark Susan
Publication year - 1994
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950160612
Subject(s) - 5 methylcytosine , dna methylation , cytosine , biology , dna , bisulfite sequencing , dna sequencing , genetics , genomic dna , genome , sequencing by ligation , gene , computational biology , genomic library , base sequence , gene expression
Abstract To analyse the biological role of 5‐methylation of cytosine residues in DNA requires precise and efficient methods for detecting individual 5‐methylcytosines (5‐MeCs) in genomic DNA. The methods developed over the past decade rely on either differential enzymatic or chemical cleavage of DNA, or more recently on differential sensitivity to chemical conversion of one base to another. The most commonly used methods for studying the methylation profile of DNA, including the bisulphite base‐conversion method, are reviewed.