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The RAD6 DNA repair pathway in Saccharomyces cerevisiae : What does it do, and how does it do it?
Author(s) -
Lawrence Christopher
Publication year - 1994
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950160408
Subject(s) - saccharomyces cerevisiae , mutagenesis , biology , dna , dna repair , genetics , mechanism (biology) , mutation , dna damage , yeast , microbiology and biotechnology , gene , philosophy , epistemology
Abstract The RAD6 pathway of budding yeast, Saccharomyces cerevisiae , is responsible for a substantial fraction of this organism's resistance to DNA damage, and also for induced mutagenesis. The pathway appears to incorporate two different recovery processes, both regulated by RAD6. The error‐prone recovery prcess accounts for only a small amount of RAD6 ‐dependent resistance, but probably all induced mutagenesis. The underlying mechanism, for error‐prone recovery is very likely to be translesion synthesis. The error‐free recovery process accounts for most of RAD6 ‐dependent resistace, but its mechanism is less clear; it may entail error‐free bypass by template switching and/or DNA gap filling by recombination. RAD6 regulates these activities by ubiquitinateins, and the roles they play in error‐free and error‐prone recovery, have not yet been established.