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SV40 DNA replication intermediates: Analysis of drugs which target mammalian DNA replication
Author(s) -
Snapka Robert M.,
Permana Paskasari A.
Publication year - 1993
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950150208
Subject(s) - origin recognition complex , replication factor c , control of chromosome duplication , replicon , eukaryotic dna replication , pre replication complex , dna replication , biology , licensing factor , dna replication factor cdt1 , replication (statistics) , dna , origin of replication , replication protein a , genetics , virology , dna binding protein , gene , plasmid , transcription factor
The simian virus 40 chromosome, a model for the mammalian replicon, is a uniquely powerful system for the study of drugs and treatments which target enzymes of the mammalian replication apparatus. High resolution gel electrophoretic analysis of normal and aberrant viral replication intermediates can be used effectively to understand the molecular events of replication failure. These events include breakage of replication forks, aberrant topoisomerase action, failure to separate daughter chromosomes, protein‐DNA crosslinking, single and double strand DNA breakage, alterations in topology and inactivation of replication intermediates. The SV40 replication system can also be used to study the recombinational events which often follow drug‐induced replication failure.