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Gene targeting and gene trap screens using embryonic stem cells: New approaches to mammalian development
Author(s) -
Joyner Alexandra L.
Publication year - 1991
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950131206
Subject(s) - biology , chimera (genetics) , germline , homologous recombination , embryonic stem cell , gene targeting , enhancer trap , gene , genetics , gastrulation , stem cell , enhancer , mutant , microbiology and biotechnology , gene expression
Mouse embryonic stem cell lines offer an attractive route for introducing rare genetic alternations into the gene pool since the cells can be pre‐screened in culture and the mutations then transmitted into the germline through chimera production. Two applications of this technique seem ideally suited for a genetic analysis of development are enhancer and gene trap screens for loci expressed during gastrulation and production of targeted mutations using homologous recombination. These approaches should greatly increase the number of mouse developmental mutants available and help to elucidate the genetic hierarchy controlling embryogenesis.