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Functional and dynamic aspects of the mammalian nucleolus
Author(s) -
Scheer Ulrich,
Benavente Ricardo
Publication year - 1990
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950120104
Subject(s) - nucleolus , ribosome biogenesis , rna polymerase i , ribosome , transcription (linguistics) , ribosomal rna , biology , ribosomal dna , microbiology and biotechnology , ribosomal protein , rna , genetics , gene , rna polymerase , phylogenetics , linguistics , philosophy , cytoplasm
Nucleoli are the sites of ribosome biogenesis. Transcription of the ribosomal RNA genes as well as processing and initial packaging of their transcripts with ribosomal and non‐ribosomal proteins all occur within the nucleolus in an ordered manner and under defined topological conditions. Components of the nucleolus have been localized by immunocytochemistry and their functional aspects investigated by microinjection of antibodies directed against the enzyme responsible for rDNA transcription, RNA polymerase I. The role of nascent transcripts in postmitotic formation of nucleoli will be discussed.