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Initiation of eukaryotic DNA replication in vitro
Author(s) -
Stillman Bruce
Publication year - 1988
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950090205
Subject(s) - eukaryotic dna replication , origin recognition complex , control of chromosome duplication , licensing factor , pre replication complex , biology , dna replication , replication factor c , semiconservative replication , origin of replication , minichromosome maintenance , replication (statistics) , dna replication factor cdt1 , genetics , microbiology and biotechnology , dna , virology
Recent advances in our understanding of the mechanism and regulation of eukaryotic DNA replication have been expedited by the use of cell‐free systems capable of initiation of DNA replication. The system capable of replicating plasmid DNAs containing the SV40 origin of DNA replication in vitro is a paradigm for studies on the replication of other virus DNAs and the replication of cellular chromosomes. This review outlines some of the contemporary issues and developments related to this complex problem.