Premium
Chromatin assembly in vitro and in vivo
Author(s) -
Dilworth Stephen M.,
Dingwall Colin
Publication year - 1988
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950090203
Subject(s) - chromatin , nucleosome , microbiology and biotechnology , in vitro , dna , chromatin remodeling , biology , in vivo , histone , computational biology , genetics , chemistry , biophysics
The assembly of nucleosomes and higher‐order chromatin structures has been extensively studied in vitro . Provided that non‐specific charge interactions are controlled, all the information for correct assembly is found to be inherent in the macromolecular components. Cellular extracts which can assemble chromatin in vitro with nucleosomes correctly spaced on the DNA have been studied in detail and also used to investigate the role of chromatin structure in transcription. However, the mechanisms of chromatin assembly in vivo are still controversial.