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Molecular mimicry of carbohydrate and protein structures by hybridoma antibodies
Author(s) -
Olsson Lennart
Publication year - 1987
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950070306
Subject(s) - antigenicity , epitope , monoclonal antibody , molecular mimicry , hybridoma technology , antigen , biology , antibody , mimicry , idiotype , microbiology and biotechnology , virology , immunology , ecology
A large proportion of tumour‐associated antigens seem to be determined by carbohydrate structures. Advances in the study of the antigenicity of cell‐surface carbohydrates have been hampered by the absence of advanced monoclonal hybridoma technology comparable to that available for the study of protein antigens. Monoclonal antibodies have been raised against a carbohydrate epitope (43–9F) that is associated with the proliferative features of squamous lung carcinomas. These were used in turn to generate anti‐idiotype antibodies with homology to 43–9F. The method and its possible applications are described, together with a procedure to detect rare cell membrane variants within large populations.