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Mutant sequences as probes of protein folding mechanisms
Author(s) -
Matthews C. Robert,
Hurle Mark R.
Publication year - 1987
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950060603
Subject(s) - folding (dsp implementation) , protein folding , mutagenesis , amino acid , mutant , chemistry , site directed mutagenesis , protein engineering , biophysics , computational biology , biochemistry , biology , enzyme , electrical engineering , gene , engineering
Mutagenesis makes it possible to examine the effect of amino acid replacements on the folding and stability of proteins. The evaluation of kinetic and equilibrium folding data using reaction coordinate diagrams allows one to determine the roles that single amino acids play in the folding mechanism.