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The amd S gene of Aspergillus nidulans : Control by multiple regulatory signals
Author(s) -
Hynes Michael J.,
Davis Meryl A.
Publication year - 1986
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950050308
Subject(s) - gene , regulator gene , biology , genetics , activator (genetics) , regulatory sequence , structural gene , gene product , regulation of gene expression , aspergillus nidulans , gene expression , mutant
The amd S gene of A. nidulans has proved extremely favourable for the isolation of mutations affecting gene regulation . Trans‐ acting regulatory genes involved in amd S induction by small molecular weight effectors have been identified – amd R (ω‐amino acids) facB (acetate) and amd A (acetate). Another gene, the areA gene, has properties expected of a major activator gene involved in nitrogen metabolite repression of amd S. All of these regulatory genes are also involved in the control of various other functions encoded by structural genes unlinked to amd S. Mutations in the 5′‐region adjacent to amd S have been isolated and allow the identification of independent cis‐acting sequences which are the target sites for the regulatory genes. The involvement of these sequences in regulatory product binding has been deduced from titration studies using transformants containing multiple copies of the 5′ sequences. A combination of genetics and molecular analysis is allowing a detailed characterization of this system.