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Regulation of enzymes in isolated plant nuclei
Author(s) -
Datta Neeraj,
Roux Stanley J.
Publication year - 1986
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950050307
Subject(s) - phosphorylation , phosphatase , enzyme , kinase , biochemistry , microbiology and biotechnology , biology , nuclear protein , protein phosphorylation , protein kinase a , chemistry , transcription factor , gene
Purified nuclei are being used as a test system to study the regulation of nuclear enzymes in plants. Regulatory agents such as light, hormones and polyamines can stimulate kinases or phosphatases that control nuclear protein phosphorylation and they can modulate the activity of as yet unidentified enzymes required for transcript synthesis and/or stabilization. This essay summarizes current findings and discusses the advantages and pitfalls of using isolated nuclei to investigate how nuclear functions are controlled.