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Splicing of messenger RNA precursors
Author(s) -
Patwardhan Satish,
Kaltwasser Gustavo,
Dimaria Peter R.,
Goldenberg Carlos J.
Publication year - 1985
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950020505
Subject(s) - rna splicing , messenger rna , rna , in vitro , microbiology and biotechnology , rna processing , biology , post transcriptional modification , alternative splicing , precursor mrna , chemistry , computational biology , biochemistry , gene
The splicing of pre‐mRNAs in vitro is accomplished by formation of RNA intermediates in a lariat form. Lariat RNAs have been recently identified in vivo supporting the validity of the proposed pathway for processing pre‐mRNAs in vitro. We have recently reported 20 a partial purification scheme for a pre‐mRNA splicing activity. Purification of the individual components and eventually reconstitution of the reaction with purified activities will firmly establish the pathways for pre‐mRNA splicing and help to elucidate the detailed biochemical mechanism of the several reactions involved in mRNA splicing.