Premium
What'S new: The role of chaotropic salts in two‐phase gene diagnosis
Author(s) -
Gillespie David,
Caranfa Mary Jo,
Bresser Joel
Publication year - 1984
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950010610
Subject(s) - chaotropic agent , chemistry , dissolution , salt (chemistry) , gene , membrane , phase (matter) , dna , biochemistry , organic chemistry
The preparation of samples for gene diagnosis is time‐consuming, labor‐intensive and costly. These problems can be overcome by dissolving a biological source in a strong solution of a chaotropic salt, then filtering the solution through an immobilizing membrane. Conditions exist for causing the selective immobilization of either DNA or mRNA. The resultant membrane is immediately ready for reaction with labeled gene probes.