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Eukaryotic cloning vectors based on bovine papilloma viruses
Author(s) -
DiMaio Daniel
Publication year - 1984
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.950010110
Subject(s) - cloning (programming) , biology , gene , recombinant dna , computational biology , vector (molecular biology) , origin of replication , eukaryotic cell , shuttle vector , genetics , molecular cloning , virology , function (biology) , genome , gene expression , computer science , programming language
The cloning of eukaryotic genes by standard recombinant DNA techniques permits their structural characterization. However, analysis of the expression properties of these genes often requires their introduction into and replication within eukaryotic cells in culture. Certain viral vectors based on the papilloma viruses may prove to be especially important in such investigations. These ‘shuttle vectors’, capable of replication in both bacterial and eukaryotic cells, have already provided several findings of interest about the relationship between eukaryotic gene structure and function. Further analysis of these vectors and their properties promises to enhance their use as investigative tools.