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Reduced representation sequencing: A success in maize and a promise for other plant genomes
Author(s) -
Barbazuk W. Brad,
Bedell Joseph A.,
Rabinowicz Pablo D.
Publication year - 2005
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.20262
Subject(s) - genome , biology , dna sequencing , gene , genetics , reference genome , whole genome sequencing , computational biology
Plant, and particularly cereal genomes, are challenging to sequence due to their large size and high repetitive DNA content. Gene‐enrichment strategies are alternative or complementary approaches to complete genome sequencing that yield, rapidly and inexpensively, useful sequence data from large and complex genomes. The maize genome is large (2.7 Gbp) and contains large amounts of conserved repetitive elements. Furthermore, the high allelic diversity found between maize inbred lines may necessitate sequencing several inbred lines in order to recover the maize “gene pool”. Two gene‐enrichment approaches, methylation filtration (MF) and high C o t (HC) sequencing have been tested in maize and their ability to sample the gene space has been examined. Combined with other genomic sequencing strategies, gene‐enriched genomic sequencing is a practical way to examine the maize gene pool, to order and orient the genic sequences on the genome, and to enable investigation of gene content of other complex plant genomes. BioEssays 27:839–848, 2005. © 2005 Wiley Periodicals, Inc.