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Palmitoylated Proteins in Plasmodium falciparum ‐Infected Erythrocytes: Investigation with Click Chemistry and Metabolic Labeling
Author(s) -
Kilian Nicole,
Zhang Yongdeng,
LaMonica Lauren,
Hooker Giles,
Toomre Derek,
Mamoun Choukri Ben,
Ernst Andreas M.
Publication year - 2020
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.201900145
Subject(s) - plasmodium falciparum , click chemistry , palmitoylation , biology , microbiology and biotechnology , confocal microscopy , plasmodium (life cycle) , fluorescence microscope , parasite hosting , malaria , chemistry , biochemistry , fluorescence , cysteine , immunology , enzyme , physics , quantum mechanics , world wide web , computer science , combinatorial chemistry
The examination of the complex cell biology of the human malaria parasite Plasmodium falciparum usually relies on the time‐consuming generation of transgenic parasites. Here, metabolic labeling and click chemistry are employed as a fast transfection‐independent method for the microscopic examination of protein S‐ palmitoylation, an important post‐translational modification during the asexual intraerythrocytic replication of P. falciparum . Applying various microscopy approaches such as confocal, single‐molecule switching, and electron microscopy, differences in the extent of labeling within the different asexual developmental stages of P. falciparum and the host erythrocytes over time are observed.