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Temporal and spatial regulation of mRNA export: Single particle RNA‐imaging provides new tools and insights
Author(s) -
Heinrich Stephanie,
Derrer Carina Patrizia,
Lari Azra,
Weis Karsten,
Montpetit Ben
Publication year - 2017
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.201600124
Subject(s) - messenger rna , rna , gene expression , biology , biogenesis , reinterpretation , cytoplasm , microbiology and biotechnology , computational biology , live cell imaging , gene , genetics , cell , physics , acoustics
The transport of messenger RNAs (mRNAs) from the nucleus to cytoplasm is an essential step in the gene expression program of all eukaryotes. Recent technological advances in the areas of RNA‐labeling, microscopy, and sequencing are leading to novel insights about mRNA biogenesis and export. This includes quantitative single molecule imaging (SMI) of RNA molecules in live cells, which is providing knowledge of the spatial and temporal dynamics of the export process. As this information becomes available, it leads to new questions, the reinterpretation of previous findings, and revised models of mRNA export. In this review, we will briefly highlight some of these recent findings and discuss how live cell SMI approaches may be used to further our current understanding of mRNA export and gene expression.