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Fluorescence correlation spectroscopy
Author(s) -
Ries Jonas,
Schwille Petra
Publication year - 2012
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.201100111
Subject(s) - fluorescence correlation spectroscopy , biomolecule , fluorescence , fluorescence spectroscopy , spectroscopy , biological system , homogeneous , measure (data warehouse) , nanotechnology , fluorescence cross correlation spectroscopy , chemistry , biophysics , computer science , biochemical engineering , materials science , biology , physics , statistical physics , engineering , optics , data mining , quantum mechanics
Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to various biological systems such as simple homogeneous solutions, cells, artificial, or cellular membranes and whole organisms. Here, we introduce the basic principle of FCS, discuss its application to biological questions as well as its limitations and challenges, present an overview of novel technical developments to overcome those challenges, and conclude with speculations about the future applications of fluorescence fluctuation spectroscopy.