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Macromolecular complexes that unwind nucleic acids
Author(s) -
von Hippel Peter H.,
Delagoutte Emmanuelle
Publication year - 2003
Publication title -
bioessays
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.175
H-Index - 184
eISSN - 1521-1878
pISSN - 0265-9247
DOI - 10.1002/bies.10369
Subject(s) - helicase , nucleic acid , dna , macromolecule , transcription (linguistics) , biology , rna , function (biology) , polymerase , biochemistry , computational biology , gene , chemistry , biophysics , microbiology and biotechnology , linguistics , philosophy
In this essay, we consider helicases, defined as enzymes that use the free energies of binding and hydrolysis of ATP to drive the unwinding of double‐stranded nucleic acids, and ask how they function within, and are “coupled” to, the macromolecular machines of gene expression. To illustrate the principles of the integration of helicases into such machines, we consider the macromolecular complexes that direct and control DNA replication and DNA‐dependent RNA transcription, and use these systems to illustrate how machines centered around coupled polymerase–helicase systems can be regulated by small changes in the interactions of their functional components. BioEssays 25:1168–1177, 2003. © 2003 Wiley Periodicals, Inc.