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Synthesis of diazoluminomelanin (DALM) in HL‐60 cells for possible use as a cellular‐level microwave dosimeter
Author(s) -
Bruno John G.,
Kiel Johnathan L.
Publication year - 1994
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.2250150406
Subject(s) - luminescence , materials science , intracellular , polymer , cell culture , biophysics , chemistry , biochemistry , biology , optoelectronics , genetics , composite material
Abstract Chemical and bacterial synthesis of a thermochemiluminescent polymer known as diazoluminomelanin (DALM) has been previously reported. This paper focuses on the intracellular synthesis of aminomelanin (AM) in mammalian cell lines and subsequent DALM synthesis from this core molecule. B16 melanoma cells, HL‐60 myeloid leukemia cells, and RAW 264.7 macrophages show AM and DALM production. Macroscopic image analysis of HL‐60 cell lysates containing DALM using the Quantitative Luminescence Imaging System (QLIS) showed increased chemiluminescence (CL) with increased microwave power input and increased temperature. This work represents a first step toward the goal of microscopic radiofrequency dosimetry of individual DALM‐loaded cells using image analysis. © 1994 Wiley‐Liss, Inc.