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Dose dependence of acetylcholinesterase activity in neuroblastoma cells exposed to modulated radio‐frequency electromagnetic radiation
Author(s) -
Dutta S. K.,
Das K.,
Ghosh B.,
Blackman C. F.
Publication year - 1992
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.2250130407
Subject(s) - acetylcholinesterase , electromagnetic radiation , radio frequency , radiation , physics , extremely low frequency , electromagnetic field , nuclear magnetic resonance , optics , electrical engineering , engineering , enzyme , quantum mechanics
Radio‐frequency electromagnetic radiation (RFR) at 915 and at 147 MHz, when sinusoidally amplitude modulated (AM) at 16 Hz, has been shown to enhance release of calcium ions from neuroblastoma cells in culture. The dose‐response relation is unusual, consisting of two power‐density “windows” in which enhanced efflux occurs, separated by power‐density regions in which no effect is observed. To explore the physiological importance of these findings, we have examined the impact of RFR exposure on a membrane‐bound enzyme, acetylcholinesterase (AChE), which is intimately involved with the acetylcholine (ACh) neurotransmitter system. Neuroblastoma cells (NG108), exposed for 30 min to 147‐MHz radiation, AM at 16 Hz, demonstrated enhanced AChE activity, as assayed by a procedure using 14 C‐labeled ACh. Enhanced activity was observed within a time window between 7.0 and 7.5 h after the cells were plated and only when the exposure occurred at power densities identified in a previous report as being effective for altering the release of calcium ions. Thus RFR affects both calcium‐ion release and AChE activity in nervous system‐derived cells in culture in a common dose‐dependent manner. 1992 Wiley‐Liss, Inc.