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Japanese encephalitis virus (JEV): Potentiation of lethality in mice by microwave radiation
Author(s) -
Lange David G.,
Sedmak J.
Publication year - 1991
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.2250120603
Subject(s) - lethality , hypercarbia , virus , long term potentiation , japanese encephalitis , pinocytosis , virology , microwave , biology , encephalitis , chemistry , medicine , toxicology , endocrinology , cell , biochemistry , receptor , endocytosis , acidosis , physics , quantum mechanics
Abstract The expression of Japanese Encephalitis Virus (JEV) lethality in mice requires entry of the virus into the central nervous system. This entry is presumably through the capillary endothelial cells (CEC), because entry between CECs is inhibited by bands of circumferential tight‐junctions. A viremic stage occurs during the first 4 to 5 days after JEV administration in mice, and both microwave radiation (2.45‐GHz, continuous wave, 10‐min exposure) and hypercarbia were employed to increase CEC permeability to JEV. Exposure to microwaves at power densities of 10–50 mW/cm 2 resulted in a dose‐dependent increase in JEV‐induced lethality. Mice did not become tolerant or sensitized to microwave potentiation of JEV‐induced mortality because 4 daily exposures at 10 or 50 mW/cm 2 (SARS, ∼ 24–98 W/kg) did not alter the lethality pattern to subsequent microwave radiation of JEV‐exposed animals. Similarly, hypercarbia (5, 10, and 20% CO 2 ) was observed to produce a dose‐dependent increase in JEV‐induced lethality. Both microwave radiation and hypercarbia are thought to promote pinocytosis in CNS capillary endothelial cells. This may be one mechanism by which they enhance JEV‐induced lethality in adult Swiss‐Cox mice.