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Calcium uptake by leukemic and normal T‐lymphocytes exposed to low frequency magnetic fields
Author(s) -
Lyle Daniel B.,
Wang Xinghua,
Ayotte Robert D.,
Sheppard Asher R.,
Adey W. Ross
Publication year - 1991
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.2250120303
Subject(s) - calcium , spleen , cytotoxic t cell , incubation , chemistry , magnetic field , nuclear magnetic resonance , endocrinology , medicine , biology , immunology , in vitro , biochemistry , physics , quantum mechanics
Calcium‐ion uptake by normal and leukemia lymphocytes increased during a 30‐min exposure to a 13.6 Hz, sinusoidal magnetic field at 20 μT peak. The time‐varying field was horizontal and parallel to a 16.5 μT component of the ambient static magnetic field. The uptake of 45 Ca 2+ increased 102% in a line of murine, cytotoxic T‐lymphocytes (C57B1/6‐derived CTLL‐1), increased 126% in freshly‐isolated spleen lymphocytes (C57B1/6 mice), and increased 75% in a line of lymphoma cells (C57B1/6‐derived EL4). In contrast, there was no effect when the same field was applied for 30 min immediately before—as opposed to during—incorporation of calcium ions. When spleen lymphocytes were exposed during incubation with 45 Ca 2+ to a 60 Hz magnetic field at 20 μT peak, a small but statistically significant increase (37%) in uptake of the labeled ions occurred. These results indicate that weak, alternating magnetic fields might affect calcium‐dependent functions of normal and leukemic lymphocytes.