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Immunological effects of amplitude‐modulated radio frequency radiation: B lymphocyte capping
Author(s) -
Sultan Michel F.,
Cain Charles A.,
Tompkins Wayne A. F.
Publication year - 1983
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.2250040206
Subject(s) - irradiation , immunofluorescence , lymphocyte , chemistry , biophysics , amplitude , radiation , in vitro , antibody , microbiology and biotechnology , materials science , immunology , biology , optics , biochemistry , physics , nuclear physics
B lymphocytes collected from normal ICR Swiss mouse spleens were exposed in vitro in a Crawford cell to 147‐MHz radiofrequency (RF) radiation, amplitude modulated by a 9‐, 16‐, or 60‐Hz sine wave. The power densities ranged between 0.11 and 48 mW/cm 2 . The irradiated samples and the controls were maintained at 37 °C or 42 °C, with temperature variations less than 0.1 °C. Immediately after a 30‐minute exposure, the distribution of antigen‐antibody (AgAb) complexes on the cell surface was evaluated at 37 °C by immunofluorescence. Under normal conditions (37 °C, no RF), AgAb complexes are regrouped into a polar cap by an energy‐dependent process. Our results demonstrate that the irradiated cells and the nonirradiated controls capped AgAb complexes equally well after exposure at 37 °C. Capping was equally inhibited at 42 °C in both the controls and irradiated cells. No statistically significant differences in capping were observed between the RF‐exposed and control samples at any of the modulation frequencies and power densities employed as long as both preparations were maintained at the same temperature.