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In Vitro Evaluation of the Effect of Stimulation with Magnetic Fields on Chondrocytes
Author(s) -
Escobar Juan Felipe,
VacaGonzález Juan Jairo,
Guevara Johana Maria,
Vega Jose Félix,
Hata Yoshie Adriana,
GarzónAlvarado Diego Alexander
Publication year - 2020
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.22231
Subject(s) - bioelectromagnetics , stimulation , chondrocyte , in vitro , glycosaminoglycan , extracellular matrix , cell growth , cartilage , microbiology and biotechnology , chemistry , cell culture , biophysics , biomedical engineering , immunology , medicine , biology , neuroscience , anatomy , biochemistry , physics , magnetic field , genetics , quantum mechanics
Magnetic fields (MFs) have been used as an external stimulus to increase cell proliferation in chondrocytes and extracellular matrix (ECM) synthesis of articular cartilage. However, previously published studies have not shown that MFs are homogeneous through cell culture systems. In addition, variables such as stimulation times and MF intensities have not been standardized to obtain the best cellular proliferative rate or an increase in molecular synthesis of ECM. In this work, a stimulation device, which produces homogeneous MFs to stimulate cell culture surfaces was designed and manufactured using a computational model. Furthermore, an in vitro culture of primary rat chondrocytes was established and stimulated with two MF schemes to measure both proliferation and ECM synthesis. The best proliferation rate was obtained with an MF of 2 mT applied for 3 h, every 6 h for 8 days. In addition, the increase in the synthesis of glycosaminoglycans was statistically significant when cells were stimulated with an MF of 2 mT applied for 5 h, every 6 h for 8 days. These findings suggest that a stimulation with MFs is a promising tool that could be used to improve in vitro treatments such as autologous chondrocyte implantation, either to increase cell proliferation or stimulate molecular synthesis. Bioelectromagnetics. 2020;41:41–51 © 2019 Bioelectromagnetics Society