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Adverse and beneficial effects in Chinese hamster lung fibroblast cells following radiofrequency exposure
Author(s) -
Sannino Anna,
Zeni Olga,
Romeo Stefania,
Massa Rita,
Scarfi Maria Rosaria
Publication year - 2017
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.22034
Subject(s) - chinese hamster , fibroblast , mitomycin c , micronucleus test , micronucleus , clastogen , hamster , mutagen , cytotoxicity , chemistry , toxicity , andrology , pharmacology , cell culture , medicine , toxicology , microbiology and biotechnology , carcinogen , biology , biochemistry , in vitro , surgery , genetics
In this study, the effect of radiofrequency (RF) exposure to 1950 MHz, Universal Mobile Telecommunication System signal, was investigated in Chinese hamster lung fibroblast cell line (V79). Genotoxic and cytotoxic effects of 20‐h exposure at specific absorption rate (SAR) values from 0.15 W/kg to 1.25 W/kg were measured by means of cytokinesis‐block micronucleus (MN) assay. Exposure was carried out blinded under strictly controlled conditions of dosimetry and temperature. The effect of RF exposure alone at four SAR values was tested, that is, 0.15, 0.3, 0.6, and 1.25 W/kg. A statistically significant increase in MN frequency was found in cultures exposed to 0.15 and 0.3 W/kg ( P < 0.05) compared to sham‐exposed ones, in the absence of cytotoxicity. SAR values of 0.6 and 1.25 W/kg did not exert any effect. Moreover, to evaluate the ability of RF to exert protective effects with respect to a chemical mutagen, cell cultures were also pre‐exposed for 20 h at 0.3 or 1.25 W/kg, and then treated with 500 ng/ml of mitomycin‐C (MMC). A significant reduction in the frequency of MN was detected in cultures pre‐exposed to 1.25 W/kg compared to cultures treated with MMC alone ( P < 0.05), indicating induction of adaptive response. Such a decrease was not induced by pre‐exposure at 0.3 W/kg SAR. Taken together, our results indicated that V79 is a sensitive cell model to evidence either adverse or beneficial effects of RF exposure, depending on experimental conditions applied. Bioelectromagnetics. 38:245–254, 2017. © 2017 Wiley Periodicals, Inc.