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Pulsed magnetic stimulation modifies amplitude of action potentials in vitro via ionic channels‐dependent mechanism
Author(s) -
Ahmed Zaghloul,
Wieraszko Andrzej
Publication year - 2015
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.21917
Subject(s) - veratridine , bioelectromagnetics , sodium channel , biophysics , tetrodotoxin , chemistry , stimulation , compound muscle action potential , mechanism of action , electrophysiology , resting potential , sodium , nuclear magnetic resonance , pharmacology , in vitro , membrane potential , biochemistry , medicine , biology , physics , electromagnetic field , organic chemistry , quantum mechanics
This paper investigates the influence of pulsed magnetic fields (PMFs) on amplitude of evoked, compound action potential (CAP) recorded from the segments of sciatic nerve in vitro. PMFs were applied for 30 min at frequency of 0.16 Hz and intensity of 15 mT. In confirmation of our previous reports, PMF exposure enhanced amplitude of CAPs. The effect persisted beyond PMF activation period. As expected, CAP amplitude was attenuated by antagonists of sodium channel, lidocaine, and tetrodotoxin. Depression of the potential by sodium channels antagonists was reversed by subsequent exposure to PMFs. The effect of elevated potassium concentration and veratridine on the action potential was modified by exposure to PMFs as well. Neither inhibitors of protein kinase C and protein kinase A, nor known free radicals scavengers had any effects on PMF action. Possible mechanisms of PMF action are discussed. Bioelectromagnetics. 36:386–397, 2015. © 2015 Wiley Periodicals, Inc.