Premium
Pre‐exposure of neuroblastoma cell line to pulsed electromagnetic field prevents H 2 O 2 ‐induced ROS production by increasing MnSOD activity
Author(s) -
Osera Cecilia,
Amadio Marialaura,
Falone Stefano,
Fassina Lorenzo,
Magenes Giovanni,
Amicarelli Fernanda,
Ricevuti Giovanni,
Govoni Stefano,
Pascale Alessia
Publication year - 2015
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.21900
Subject(s) - bioelectromagnetics , oxidative stress , reactive oxygen species , superoxide dismutase , cell culture , acute exposure , microbiology and biotechnology , chemistry , neuroscience , biology , biophysics , endocrinology , biochemistry , electromagnetic field , genetics , physics , quantum mechanics
Electromagnetic fields (EMFs) have been linked to increased risk of cancers and neurodegenerative diseases; however, EMFs can also elicit positive effects on biological systems, and redox status seems crucially involved in EMF biological effects. This study aimed to assess whether a short and repeated pulsed EMF (PEMF) could trigger adaptive responses against an oxidative insult in a neuronal cellular model. We found that a 40 min overall (four times a week, 10 min each) pre‐exposure to PEMF did not affect major physiological parameters and led to a significant increase of Mn‐dependent superoxide dismutase activity in the human neuroblastoma SH‐SY5Y cell line. In addition, we found PEMF‐pre‐exposed cells exhibited decreased reactive oxygen species production following a 30 min H 2 O 2 challenge, with respect to non pre‐exposed cells. Our findings might provide new insights on the role played by short and repeated PEMF stimulations in the enhancement of cellular defenses against oxidative insults. Although studies in normal neuronal cells would be useful to further confirm our hypothesis, we suggest that specific PEMF treatments may have potential biological repercussions in diseases where oxidative stress is implicated. Bioelectromagnetics. Bioelectromagnetics. 36:219–232, 2015. © 2015 Wiley Periodicals, Inc.