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Combined effect of X‐ray radiation and static magnetic fields on reactive oxygen species in rat lymphocytes in vitro
Author(s) -
Politański Piotr,
Rajkowska Elźbieta,
Brodecki Marcin,
Bednarek Andrzej,
Zmyślony Marek
Publication year - 2013
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.21767
Subject(s) - bioelectromagnetics , reactive oxygen species , fluorescence , oxygen , earth's magnetic field , in vitro , radiation , incubation , biophysics , chemistry , magnetic field , intracellular , nuclear magnetic resonance , analytical chemistry (journal) , biology , optics , physics , biochemistry , environmental chemistry , quantum mechanics , organic chemistry
The aim of this study was to investigate the effect of static magnetic fields (SMF) on reactive oxygen species induced by X‐ray radiation. The experiments were performed on lymphocytes from male albino Wistar rats. After exposure to 3 Gy X‐ray radiation (with a dose rate of 560 mGy/min) the measurement of intracellular reactive oxygen species in lymphocytes, using a fluorescent probe, was done before exposure to the SMF, and after 15 min, 1 and 2 h of exposure to the SMF or a corresponding incubation time. For SMF exposure, 0 mT (50 µT magnetic field induction opposite to the geomagnetic field) and 5 mT fields were chosen. The trend of SMF effects for 0 mT was always opposite that of 5 mT. The first one decreased the rate of fluorescence change, while the latter one increased it. Bioelectromagnetics 34:333–336, 2013. © 2012 Wiley Periodicals, Inc.