Premium
Time‐varying magnetic fields of 60 Hz at 7 mT induce DNA double‐strand breaks and activate DNA damage checkpoints without apoptosis
Author(s) -
Kim Jiyeon,
Yoon Yeojun,
Yun Seungho,
Park Gwan Soo,
Lee Hae June,
Song Kiwon
Publication year - 2012
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.21697
Subject(s) - dna damage , dna , apoptosis , microbiology and biotechnology , reactive oxygen species , intracellular , programmed cell death , biophysics , chemistry , biology , biochemistry
The potential genotoxic effect of a time‐varying magnetic field (MF) on human cells was investigated. Upon continuous exposure of human primary fibroblast and cervical cancer cells to a 60 Hz MF at 7 mT for 10–60 min, no significant change in cell viability was observed. However, deoxyribonucleic acid (DNA) double‐strand breaks (DSBs) were detected, and the DNA damage checkpoint pathway was activated in these cells without programmed cell death (called apoptosis). The exposure of human cells to a 60 Hz MF did not induce intracellular reactive oxygen species (ROS) production, suggesting that the observed DNA DSBs are not directly caused by ROS. We also compared the position and time dependency of DNA DSBs with numerical simulation of MFs. The Lorentz force and eddy currents in these experiments were numerically calculated to investigate the influence of each factor on DNA DSBs. The DNA DSBs mainly occurred at the central region, where the MF was strongest, after a 30‐min exposure. After 90 min, however, the amount of DNA DSBs increased rapidly in the outer regions, where the eddy current and Lorentz force were strong. Bioelectromagnetics 33:383–393, 2012. © 2011 Wiley Periodicals, Inc.