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Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field
Author(s) -
Polidori Emanuela,
Zeppa Sabrina,
Potenza Lucia,
Martinelli Chiara,
Colombo Evelin,
Casadei Lucia,
Agostini Deborah,
Sestili Piero,
Stocchi Vilberto
Publication year - 2012
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20686
Subject(s) - umbilical vein , gene , gene expression , cell , biology , cell growth , microbiology and biotechnology , human umbilical vein endothelial cell , dna damage , cell division , oxidative stress , dna , genetics , biochemistry , in vitro
Abstract In a previous investigation we reported that exposure to a moderate (300 mT) static magnetic field (SMF) causes transient DNA damage and promotes mitochondrial biogenesis in human umbilical vein endothelial cells (HUVECs). To better understand the response of HUVECs to the 300 mT SMF, a high‐quality subtracted cDNA library representative of genes induced in cells after 4 h of static magnetic exposure was constructed. The global gene expression profile showed that several genes were induced after the SMF exposure. The characterized clones are involved in cell metabolism, energy, cell growth/division, transcription, protein synthesis, destination and storage, membrane injury, DNA damage/repair, and oxidative stress response. Quantitative real‐time polymerase chain reaction (qRT‐PCR) experiments were performed at 4 and 24 h on four selected genes. Their expression profiles suggest that HUVEC's response to SMF exposure is transient. Furthermore, compared to control cells, an up‐regulation of several genes involved in cell growth and division was observed. This up‐regulation is likely to be the cause of the slight, but significant, increase in cell proliferation at 12 h post‐treatment. These results provide additional support to the notion that SMFs may be harmless to human health, and could support the rationale for their possible use in medical treatments. Bioelectromagnetics 33:65–74, 2012. © 2011 Wiley Periodicals, Inc.