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No effects of pulsed electromagnetic fields on expression of cell adhesion molecules (integrin, CD44) and matrix metalloproteinase‐2/9 in osteosarcoma cell lines
Author(s) -
Zhang Daguang,
Pan Xin,
Ohno Shuichiro,
Osuga Tomoaki,
Sawada Shigeyuki,
Sato Keiji
Publication year - 2011
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20647
Subject(s) - cd44 , osteosarcoma , cell adhesion molecule , metastasis , matrix metalloproteinase , cancer research , integrin , cell culture , chemistry , pathology , cell adhesion , cell , extracellular matrix , microbiology and biotechnology , medicine , biology , immunology , cancer , biochemistry , genetics
Pulsed electromagnetic fields (PEMF) could enhance the cytocidal effects of chemotherapeutic drugs on malignant tumor cell lines, but metastasis effects of PEMF on tumor cells have not been investigated. We investigated the effects of PEMF exposure on the expression levels of some metastasis‐related molecules, including integrin α subunits (α1, α2, α3, α4, α5, α6, αv), integrin β subunits (β1, β2, β3, β4), CD44, and matrix metalloproteinase‐2/9 (MMP‐2/9) in four human osteosarcoma cell lines (HOS, MG‐63, SAOS‐2, NY) and two mouse osteosarcoma cell lines (DOS, LM8) by using FACScan analysis, gelatin zymography, and Western blot analysis. Our results indicate that PEMF exposure has no effect on the expression of some molecules that are associated with tumor cell invasion and metastasis, and therefore suggest that PEMF exposure may be safely applied to chemotherapy for osteosarcoma. Bioelectromagnetics 32:463–473, 2011. © 2011 Wiley‐Liss, Inc.