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Effects of homogeneous and inhomogeneous static magnetic fields combined with gamma radiation on DNA and DNA repair
Author(s) -
Kubinyi Györgyi,
Zeitler Zsuzsanna,
Thuróczy György,
Juhász Péter,
Bakos József,
Sinay Hanna,
László János
Publication year - 2010
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20577
Subject(s) - irradiation , comet assay , dna damage , homogeneous , significant difference , dna , bioelectromagnetics , radiation , materials science , microbiology and biotechnology , biophysics , analytical chemistry (journal) , magnetic field , chemistry , biology , optics , physics , medicine , chromatography , biochemistry , nuclear physics , thermodynamics , quantum mechanics
Abstract The aim of this study was to reveal whether static magnetic fields (SMFs) influence the repair of radiation‐damaged DNA on leukocytes or has any effect on DNA. After 4 Gy of 60 Co‐γ irradiation, some of the samples were exposed to inhomogeneous SMFs with a lateral magnetic flux density gradient of 47.7, 1.2, or 0.3 T/m by 10 mm lateral periodicity, while other samples were exposed to homogeneous SMF of 159.2 ± 13.4 mT magnetic flux density for a time period of 0.5 min, 1, 2, 4, 6, 18, 20, or 24 h. Another set of samples was exposed to the aforementioned SMFs before gamma irradiation. The following three groups were examined: (i) exposed to SMF only, (ii) exposed to SMF following irradiation by 60 Co‐γ, and (iii) exposed to SMF before 60 Co‐γ irradiation. The analysis of the DNA damage was made by single‐cell gel electrophoresis technique (comet assay). Statistically significant differences were found at 1 h (iSMF), 4 h (hSMF), and 18 h (hSMF) if samples were exposed to only SMF, compared to control. When the SMF exposure followed the 60 Co‐γ irradiation, statistically significant differences were found at 1 h (iSMF) and 4 h (hSMF). If exposure to SMF preceded 60 Co‐γ irradiation, no statistically significant difference was found compared to 4 Gy gamma‐irradiated group. Bioelectromagnetics 31:488–494, 2010. © 2010 Wiley‐Liss, Inc.