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Enhancement of the hydrolysis activity of F 0 F 1 ‐ATPases using 60 Hz magnetic fields
Author(s) -
Chen Chuanfang,
Cui Yuanbo,
Yue Jiachang,
Huo Xiaolin,
Song Tao
Publication year - 2009
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20509
Subject(s) - magnetic field , atpase , hydrolysis , atp hydrolysis , bioelectromagnetics , chemistry , intensity (physics) , membrane , nuclear magnetic resonance , biophysics , enzyme , biochemistry , physics , biology , optics , quantum mechanics
Abstract The effects of extremely low frequency (ELF) magnetic fields on membrane F 0 F 1 ‐ATPase activity have been studied. When the F 0 F 1 ‐ATPase was exposed to 60 Hz magnetic fields of different magnetic intensities, 0.3 and 0.5 mT magnetic fields enhanced the hydrolysis activity, whereas 0.1 mT exposure caused no significant changes. Even if the F 0 F 1 ‐ATPase was inhibited by N , N ‐dicyclohexylcarbodiimide, its hydrolysis activity was enhanced by a 0.5 mT 60 Hz magnetic field. Moreover, when the chromatophores which were labeled with F‐DHPE were exposed to a 0.5 mT, 60 Hz magnetic field, it was found that the pH of the outer membrane of the chromatophore was unchanged, which suggested that the magnetic fields used in this work did not affect the activity of F0. Taken together, our results show that the effects of magnetic fields on the hydrolysis activity of the membrane F 0 F 1 ‐ATPases were dependent on magnetic intensity and the threshold intensity is between 0.1 and 0.3 mT, and suggested that the F1 part of F 0 F 1 ‐ATPase may be an end‐point affected by magnetic fields. Bioelectromagnetics 30:663–668, 2009. © 2009 Wiley‐Liss, Inc.