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Real‐time measurement of cytosolic free calcium concentration in HL‐60 cells during static magnetic field exposure and activation by ATP
Author(s) -
Belton Michelle,
Commerford Kristy,
Hall Justin,
Prato Frank S.,
Carson Jeffrey J.L.
Publication year - 2008
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20409
Subject(s) - calcium , bioelectromagnetics , cytosol , biophysics , calcium signaling , membrane potential , chemistry , biochemistry , biology , magnetic field , enzyme , physics , organic chemistry , quantum mechanics
Calcium ions are involved in a number of important signal transduction pathways in cells. Cytosolic calcium concentration ([Ca 2+ ] c ) can be affected by the activation of Ca 2+ channels through the action of ligands such as ATP. The response of [Ca 2+ ] c to ligands may be affected by external factors like magnetic fields. The purpose of this study was to determine if exposure to a static magnetic field (SMF) for 800 s altered the [Ca 2+ ] c response to ATP in undifferentiated HL‐60 cells. We sham exposed or field exposed fura‐2 loaded HL‐60 cells to a SMF of 1, 10, and 100 mT. Cells were activated with ATP 300 s into the exposure. The level of [Ca 2+ ] c was followed before, during, and after field or sham exposure with a ratiometric fluorescence spectroscopy system. It was found that high concentrations of ATP resulted in greater [Ca 2+ ] c responses, but faster recovery to near basal levels. The application of 1, 10, or 100 mT SMF did not affect the [Ca 2+ ] c response to ATP. Future work could examine the effect of a longer SMF exposure on the [Ca 2+ ] c response to ATP. Longer exposures might provide sufficient time for morphological changes in the plasma membrane to occur. Bioelectromagnetics 29:439–446, 2008. © 2008 Wiley‐Liss, Inc.