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Pulsed electric field reduces the permeability of potato cell wall
Author(s) -
Galindo Federico Gómez,
Vernier P. Thomas,
Dejmek Petr,
Vicente António,
Gundersen Martin A.
Publication year - 2008
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20394
Subject(s) - electric field , bioelectromagnetics , sodium azide , permeability (electromagnetism) , biophysics , cell permeability , diffusion , nuclear magnetic resonance , chemistry , biomedical engineering , materials science , electromagnetic field , biochemistry , biology , medicine , physics , quantum mechanics , membrane , thermodynamics
The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1‐43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1‐43 and microscopic examination. Our results show a slower diffusion of FM1‐43 in the electropulsed tissue when compared with that in the non‐pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H 2 O 2 and blocked by sodium azide, an inhibitor of the production of H 2 O 2 by peroxidases. Bioelectromagnetics 29:296–301, 2008. © 2007 Wiley‐Liss, Inc.