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In vitro effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential and motility of human spermatozoa
Author(s) -
Falzone Nadia,
Huyser Carin,
Fourie Francois,
Toivo Tim,
Leszczynski Dariusz,
Franken Daniel
Publication year - 2008
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20390
Subject(s) - sperm , motility , specific absorption rate , sperm motility , membrane potential , andrology , non ionizing radiation , biology , biophysics , chemistry , physics , microbiology and biotechnology , medicine , optics , electrical engineering , engineering , antenna (radio)
Ejaculated, density purified, human spermatozoa were exposed to pulsed 900 MHz GSM mobile phone radiation at two specific absorption rate levels (SAR 2.0 and 5.7 W/kg) and compared with controls over time. Change in sperm mitochondrial membrane potential was analysed using flow cytometry. Sperm motility was determined by computer assisted sperm analysis (CASA). There was no effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential. This was also the case for all kinematic parameters assessed at a SAR of 2.0 W/kg. However, over time, the two kinematic parameters straight line velocity (VSL) and beat‐cross frequency (BCF) were significantly impaired ( P < 0.05) after the exposure at SAR 5.7 W/kg and no exposure by time interaction was present. This result should not be ascribed to thermal effects, due to the cooling methods employed in the RF chamber and temperature control within the incubator. Bioelectromagnetics 29:268–276, 2008. © 2007 Wiley‐Liss, Inc.