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EGF receptor clustering is induced by a 0.4 mT power frequency magnetic field and blocked by the EGF receptor tyrosine kinase inhibitor PD153035
Author(s) -
Jia Caili,
Zhou Zhenjie,
Liu Renchen,
Chen Shude,
Xia Ruohong
Publication year - 2007
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20293
Subject(s) - tyrosine kinase , epidermal growth factor , receptor , biology , cell surface receptor , receptor tyrosine kinase , epidermal growth factor receptor , cytoplasm , biophysics , microbiology and biotechnology , biochemistry
Abstract Atomic force microscopy (AFM), transmission electron microscopy (TEM), and confocal laser scanning microscopy were used to investigate the effects of a 50 Hz 0.4 mT magnetic field (MF) on the clustering of purified epidermal growth factor receptors (EGFRs) and EGFRs in Chinese hamster lung (CHL) cell membrane. The results demonstrate that exposing purified EGFRs to the MF for 30 min induces receptor clustering. The peak height of apparent clusters increased from 1.42 ± 0.18 (sham‐exposed) to 3.08 ± 0.38 nm (exposed) while the mean half‐width increased from 21.7 ± 2.2 to 33.0 ± 4.0 nm. A similar effect was also observed by TEM. Treatment of purified EGFR with PD153035 (PD), an EGFR‐specific tyrosine kinase (TK) inhibitor, inhibited the MF‐induced EGFR clustering of the purified proteins, an effect also observed for the receptors in cell membrane in the absence of EGF. These results strongly suggest that the 50 Hz 0.4 mT MF interferes with the EGFR signaling pathway, most likely by interacting with the cytoplasmic TK domain. Bioelectromagnetics 28:197–207, 2007. © 2006 Wiley‐Liss, Inc.