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Effect of the electrical currents generated by the intestinal smooth muscle layers on pancreatic enzyme activity: An in vitro study
Author(s) -
Dąbek Marta,
Podgurniak Paweł,
Piedra Jose L. Valverde,
Szymańczyk Sylwia,
Filip Rafał,
WojtaszPająk Anna,
Werpachowska Eliza,
Podgurniak Malgorzata,
Pierzynowski Stefan G.
Publication year - 2007
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20263
Subject(s) - enzyme , enzyme assay , in vitro , substrate (aquarium) , exopeptidase , endopeptidase , biophysics , electrode , materials science , aminopeptidase , chemistry , biology , biochemistry , ecology , leucine , amino acid
Gut enzymes in the small intestine are exposed to extremely low electrical currents (ELEC) generated by the smooth muscle. In the present study, the in vitro tests were undertaken to evaluate the effect of these electric currents on the activity of the proteolytic pancreatic digestive enzymes. A simulator generating the typical electrical activity of pig gut was used for these studies. The electric current emitted by the simulator was transmitted to the samples, containing enzyme and its substrate, using platinum plate electrodes. All samples were incubated at 37 °C for 1 h. The changes in optical density, corresponding to enzyme activity, in samples stimulated for 1 h with ELEC was compared with that not exposed to ELEC. The obtained results show that the electrical current with the characteristics of the myoelectrical migrating complex (MMC) has an influence on pancreatic enzyme activity. Increased endopeptidase and reduced exopeptidase activity was noticed in samples treated with ELEC. This observation can be of important as analyzed factors which can alter enzymatic activity of the gut, can thus also affect feed/food digestibility. Bioelectromagnetics 28:275–280, 2007. © 2007 Wiley‐Liss, Inc.