Premium
Real‐time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle
Author(s) -
McCreary Cheryl R.,
Dixon S. Jeffrey,
Fraher Laurence J.,
Carson Jeffrey J.L.,
Prato Frank S.
Publication year - 2006
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20248
Subject(s) - jurkat cells , cytosol , calcium , cell cycle progression , cell cycle , stimulation , apoptosis , biophysics , null cell , chemistry , microbiology and biotechnology , t cell , biology , biochemistry , immunology , endocrinology , enzyme , immune system , organic chemistry , gene
Extremely low frequency magnetic fields (ELF MF) have been reported to alter a number of cell signaling pathways, including those involved in proliferation, differentiation and apoptosis where cytosolic free calcium ([Ca 2+ ] c ) plays an important role. To better understand the biological conditions under which ELF MF exposure might alter [Ca 2+ ] c , we measured [Ca 2+ ] c by ratiometric fluorescence spectrophotometry during exposure to ELF MF in Jurkat E6.1 cells synchronized to different phases of the cell cycle. Suspensions of cells were exposed either to a near zero MF (Null) or a 60 Hz, 100 µT sinusoidal MF superimposed upon a collinear 78.1 µT static MF (AC + DC). An initial series of experiments indicated that the maximum increase in [Ca 2+ ] c above baseline after stimulation with anti‐CD3 was significantly higher in samples exposed to AC + DC ( n = 30) compared to Null ( n = 30) with the largest difference in G2‐M enriched samples. However, in a second study with G2‐M enriched cells, samples treated with AC + DC ( n = 17) were not statistically different from Null‐treated samples ( n = 27). Detailed analysis revealed that the dynamics in [Ca 2+ ] c before and after stimulation with anti‐CD3 were dissimilar between Null samples from each study. From the results, we concluded (i) that the ELF MF increased [Ca 2+ ] c during an antibody‐induced signaling event, (ii) that the ELF MF effect did not depend to a large degree on cell cycle, and (iii) that a field‐related change in [Ca 2+ ] c signaling appeared to correlate with features in the [Ca 2+ ] c dynamics. Future work could evaluate [Ca 2+ ] c dynamics in relation to the phase of the cell cycle and inter‐study variation, which may reveal factors important for the observation of real‐time effects of ELF MF on [Ca 2+ ] c . Bioelectromagnetics 27:354–364, 2006. © 2006 Wiley‐Liss, Inc.