Real‐time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle

Extremely low frequency magnetic fields (ELF MF) have been reported to alter a number of cell signaling pathways, including those involved in proliferation, differentiation and apoptosis where cytosolic free calcium ([Ca 2+ ] c ) plays an important role. To better understand the biological conditions under which ELF MF exposure might alter [Ca 2+ ] c , we measured [Ca 2+ ] c by ratiometric fluorescence spectrophotometry during exposure to ELF MF in Jurkat E6.1 cells synchronized to different phases of the cell cycle. Suspensions of cells were exposed either to a near zero MF (Null) or a 60 Hz, 100 µT sinusoidal MF superimposed upon a collinear 78.1 µT static MF (AC + DC). An initial series of experiments indicated that the maximum increase in [Ca 2+ ] c above baseline after stimulation with anti‐CD3 was significantly higher in samples exposed to AC + DC ( n  = 30) compared to Null ( n  = 30) with the largest difference in G2‐M enriched samples. However, in a second study with G2‐M enriched cells, samples treated with AC + DC ( n  = 17) were not statistically different from Null‐treated samples ( n  = 27). Detailed analysis revealed that the dynamics in [Ca 2+ ] c before and after stimulation with anti‐CD3 were dissimilar between Null samples from each study. From the results, we concluded (i) that the ELF MF increased [Ca 2+ ] c during an antibody‐induced signaling event, (ii) that the ELF MF effect did not depend to a large degree on cell cycle, and (iii) that a field‐related change in [Ca 2+ ] c signaling appeared to correlate with features in the [Ca 2+ ] c dynamics. Future work could evaluate [Ca 2+ ] c dynamics in relation to the phase of the cell cycle and inter‐study variation, which may reveal factors important for the observation of real‐time effects of ELF MF on [Ca 2+ ] c . Bioelectromagnetics 27:354–364, 2006. © 2006 Wiley‐Liss, Inc.