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Effects of a 2450 MHz high‐frequency electromagnetic field with a wide range of SARs on the induction of heat‐shock proteins in A172 cells
Author(s) -
Wang J.,
Koyama S.,
Komatsubara Y.,
Suzuki Y.,
Taki M.,
Miyakoshi J.
Publication year - 2006
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20226
Subject(s) - shock (circulatory) , range (aeronautics) , covid-19 , electromagnetic field , physics , acoustics , materials science , medicine , composite material , pathology , quantum mechanics , disease , infectious disease (medical specialty)
In this study, we investigated whether exposure to 2450 MHz high‐frequency electromagnetic fields (HFEMFs) could act as an environmental insult to evoke a stress response in A172 cells, using HSP70 and HSP27 as stress markers. The cells were exposed to a 2450 MHz HFEMF with a wide range of specific absorption rates (SARs: 5–200 W/kg) or sham conditions. Because exposure to 2450 MHz HFEMF at 50–200 W/kg SAR causes temperature increases in culture medium, appropriate heat control groups (38–44 °C) were also included. The expression of HSP 70 and HSP 27, as well as the level of phosphorylated HSP 27 ( 78 Ser) (p‐HSP27), was determined by Western blotting. Our results showed that the expression of HSP 70 increased in a time and dose‐dependent manner at >50 W/kg SAR for 1–3 h. A similar effect was also observed in corresponding heat controls. There was no significant change in HSP 27 expression caused by HFEMF at 5–200 W/kg or by comparable heating for 1–3 h. However, HSP 27 phosphorylation increased transiently at 100 and 200 W/kg to a greater extent than at 40–44 °C. Phosphorylation of HSP 27 reached a maximum after 1 h exposure at 100 W/kg HFEMF. Our results suggest that exposure to a 2450 MHz HFEMF has little or no apparent effect on HSP70 and HSP27 expression, but it may induce a transient increase in HSP27 Phosphorylation in A172 cells at very high SAR (>100 W/kg). Bioelectromagnetics 27:479–486, 2006. © 2006 Wiley‐Liss, Inc.