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Effects of continuous and intermittent exposure to RF fields with a wide range of SARs on cell growth, survival, and cell cycle distribution
Author(s) -
Takashima Yoshio,
Hirose Hideki,
Koyama Shin,
Suzuki Yukihisa,
Taki Masao,
Miyakoshi Junji
Publication year - 2006
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20220
Subject(s) - bioelectromagnetics , specific absorption rate , growth rate , cell growth , cell , cell cycle , andrology , biophysics , chemistry , materials science , biology , medicine , electromagnetic field , physics , biochemistry , electrical engineering , engineering , geometry , mathematics , quantum mechanics , antenna (radio)
To examine the biological effects of radio frequency (RF) electromagnetic fields in vitro, we have examined the fundamental cellular responses, such as cell growth, survival, and cell cycle distribution, following exposure to a wide range of specific absorption rates (SAR). Furthermore, we compared the effects of continuous and intermittent exposure at high SARs. An RF electromagnetic field exposure unit operating at a frequency of 2.45 GHz was used to expose cells to SARs from 0.05 to 1500 W/kg. When cells were exposed to a continuous RF field at SARs from 0.05 to 100 W/kg for 2 h, cellular growth rate, survival, and cell cycle distribution were not affected. At 200 W/kg, the cell growth rate was suppressed and cell survival decreased. When the cells were exposed to an intermittent RF field at 300 W/kg pk , 900 W/kg pk and 1500 W/kg pk (100 W/kg mean ), no significant differences were observed between these conditions and intermittent wave exposure at 100 W/kg. When cells were exposed to a SAR of 50 W/kg for 2 h, the temperature of the medium around cells rose to 39.1 °C, 100 W/kg exposure increased the temperature to 41.0 °C, and 200 W/kg exposure increased the temperature to 44.1 °C. Exposure to RF radiation results in heating of the medium, and the thermal effect depends on the mean SAR. Hence, these results suggest that the proliferation disorder is caused by the thermal effect. Bioelectromagnetics 27:392–400, 2006. © 2006 Wiley‐Liss, Inc.