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Oxygen enhances lethal effect of high‐intensity, ultrashort electrical pulses
Author(s) -
Walker Kerfoot,
Pakhomova Olga N.,
Kolb Juergen,
Schoenbach Karl S.,
Stuck Bruce E.,
Murphy Michael R.,
Pakhomov Andrei G.
Publication year - 2006
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20200
Subject(s) - oxygen , jurkat cells , biophysics , chemistry , limiting oxygen concentration , biology , immunology , immune system , organic chemistry , t cell
The study explored the effect of ambient oxygen on mammalian cell survival after exposure to 10 ns duration, high voltage electrical pulses (nsEP, 80–90 or 120–130 kV/cm; 200–400 pulses per exposure). Cell samples were equilibrated with pure nitrogen, atmospheric air, or pure oxygen prior to the nsEP treatment and were returned to the incubator (air + 5% CO 2 ) shortly after the exposure. The experiments established that survival of hypoxic Jurkat and U937 cells exceeded that of air‐equilibrated controls about twofold ( P < .01). Conversely, saturation of the medium with oxygen prior to exposure decreased Jurkat cell survival about 1.5 times, P < .01. Attenuation of the cytotoxic effect under hypoxic conditions resembled a well‐known effect of oxygen on cell killing by sparsely ionizing radiations and may be indicative of the similarity of underlying cell damage mechanisms. Bioelectromagnetics 27:221–225, 2006. Published 2005 Wiley‐Liss, Inc.