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Effect of extremely low frequency magnetic fields on calpain activation
Author(s) -
Salamino Franca,
Minafra Roberto,
Grano Valentina,
Diano Nadia,
Mita Damiano Gustavo,
Pontremoli Sandro,
Melloni Edon
Publication year - 2006
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20176
Subject(s) - calpain , protease , biophysics , chemistry , in vivo , intracellular , extremely low frequency , calcium , low frequency , enzyme , nuclear magnetic resonance , intensity (physics) , ion , magnetic field , biochemistry , biology , physics , optics , microbiology and biotechnology , organic chemistry , quantum mechanics , astronomy
The effects of low intensity, low frequency magnetic fields (MFs) on catalytic activity of the calcium dependent protease calpain was determined following the enzyme activation both in “in vitro” and “in vivo” conditions. We have observed that a 0.3 mT MF induces a significant increase in the requirement of the protease for this metal ion. This change is detectable at low [Ca 2+ ] and disappears when the level of Ca 2+ is raised to saturating amounts. The observed effects are not due to transient MF − induced conformational changes occurring in calpain, but to direct effects of the MF on Ca 2+ ions, which become less available for the binding sites present in calpain. Altogether, these results indicate that exposure to low intensity, low frequency MFs alters the intracellular Ca 2+ “availability,” thereby modifying the related cell response. Bioelectromagnetics 27:43–50, 2006. © 2005 Wiley‐Liss, Inc.