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Effects of extremely low frequency electromagnetic fields on the adenylate kinase activity of rod outer segment of bovine retina
Author(s) -
Ravera S.,
Repaci E.,
Morelli A.,
Pepe I.M.,
Botter R.,
Beruto D.
Publication year - 2004
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.20031
Subject(s) - adenylate kinase , membrane , bioelectromagnetics , biophysics , enzyme , electromagnetic field , extremely low frequency , chemistry , enzyme assay , biochemistry , biology , physics , quantum mechanics
Extremely low frequency electromagnetic fields (ELF‐EMFs) of 75 Hz with amplitudes above a threshold of about 125 μT have a dramatic effect on the adenylate kinase (AK) activity of the rod outer segment (ROS) membranes. In fact, the ATP production by ROS membranes or by purified disk membranes placed in the field decreased by approximately 54%. The decrease in enzymatic activity was independent of the time of exposure to the field and was completely reversible. When disk membranes were solubilized with Triton or a soluble isoform of AK was used, negligible effects of the field were obtained on the enzymatic activity, suggesting that the membrane has an important role in determining the conditions for the enzyme inactivation. Bioelectromagnetics 25:545–551, 2004. © 2004 Wiley‐Liss, Inc.